īoth the techniques of static light scattering (SLS) and dynamic light scattering (DLS) are proven for their versatility in different areas. Since it is a non-destructive and non-invasive technique, it has been proven to be a significant tool, especially for the investigation and characterization of biological cells. This technique has been widely used in diverse areas like astrophysical applications, biomedical applications, nanoscience applications, application in marine issues, atmospheric applications, and astrobiological applications. This makes the light scattering technique an interesting approach to exploring small particles ranging from nanometers to micrometer range, to predict their morphology without affecting the particles. ![]() In other words, it may provide information about the morphology of the unknown scattering particles under investigation. This diagnostic tool can provide information about various parameters like the shape, size, shape distribution, size distribution, refractive index, density, and composition of the scatterers. The particles may be biological cells, nanoparticles, aerosols, atmospheric dust particles, etc. Light scattering is an important diagnostic tool for particles of different origins. ![]() The reports mostly mention the information on the virion structure only, that is, whether it is spherical, elongated, or any other shape. Thus, it becomes important to include the number of spike proteins for any investigation carried out by light scattering technique. It indicates that the number of protein spikes is proportional to the magnitude of pathogenic spread. It thus indicates that the increase in the number of spike proteins per virion increases the possibility of binding into host cells and thereby enhancing the chances of infection. It has been reported by many researchers that the coronavirus enters host cells with the help of these spikes. One important feature of these viruses is the presence of protein spikes on their surface. The investigations on various properties of coronaviruses, primarily by light scattering, may provide novel tools for their identification and analyses. Coronaviruses are a group of RNA viruses that cause a hazard to human health leading to global health issues, thereby affecting economic stability. The concerns caused by these viruses were not taken seriously until their outbreak due to their pathogenic spread and the declaration of a global pandemic. ![]() These viruses cause mild to severe respiratory diseases. ![]() So the plotting concentration on horizontal and Kc/R(θ), which is equivalent as a reciprocal number of scattering intensity, on vertical after measuring scattering intensities of variable concentrations is called Debye Plot.Since angle dependency of scattering intensity appears in the large molecular weight sample, measuring scattering intensity at variable angle(θ) provides more accuracy on molecular weight measurement and radium of gyration, which is yardstick of molecular dispersion.When measured at fixed angle, inputting expected radium of gyration, correcting as an angle dependent measurement, accuracy of molecular weight is improved.The novel coronavirus ( SARS-CoV-2) recently classified as a pandemic by the WHO, has threatened the human population worldwide. In reality, scattering intensity to be obtained depends upon concentration, too. To simplify, from the bigger particle gives the stronger scattering and the smaller particle gives weaker scattering. As a principle, molecular weight is calculated from absolute value of scattered light obtained by irradiated light into colloidal particle. Static Light Scattering Method is renowned as the convenient method to know absolute molecular weight. Molecular Weight: Static Light Scattering Method
0 Comments
Leave a Reply. |